Rabbit PK

This screening assay is used to determine the bioavailability/exposure of test compounds after two routes of administration to female rabbits.

Required from Customer

  • Test compound in powder form or preformulated
  • Dose routes—intravenous bolus (IV) and oral gavage (OG)
  • Dose (mg/kg) of test compound
  • Molecular mass (exact mass) of each test compound and its salt form
  • MSDS or handling and storage information, e.g., light sensitive, store at -20°C, etc.
  • If applicable, instructions for dose vehicle preparation


  • In-life observations on each rat
  • A table containing test compound concentrations in plasma samples at each time point
  • A table listing relative exposure (bioavailability) based on the AUC for each compound and Concentration vs. Time profiles
  • A table containing appropriate pharmacokinetic parameters for each dose route: Cmax and Tmax for each compound, and half life, clearance, and volume of distribution of each compound after IV administration, if applicable


  • Test compound in an appropriate dosing vehicle

Assay System

  • Conscious, fasted, female New Zealand White rabbits weighing between 3.0 and 5.0 kg; water is offered ad libitum

Assay Conditions

  • Three rabbits (N=3) per dose route
  • Sample blood from an auricular vessel (vein or artery) at the following time points:
    Typically predose, 5 (IV), 15, 30 min, 1, 3, and 6 hours
  • Prepare plasma from each sample and freeze
  • Determine the concentrations of test compounds in dosing solution and incurred samples using a generic LC-MS/MS method with a minimum 6 point calibration curve*
  • Non-compartmental analysis is used to determine PK parameters for each test compound

Assay QC

  • At least 60% of the calibration standards must be within ±20% of the theoretical values to accept the analytical run


  1. The results from this study are provided to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. *The analytical rigor does not include a pre-study validation, and QCs will not be used for sample analysis.
  3. If required, Absorption Systems can determine a suitable dosing vehicle and prepare it for the experiment. Alternatively, Absorption Systems can prepare the dosing vehicle if the customer provides instructions.
  4. One hour formulation prep cost has been included in the study price. Additional time required for formulation preparation will be charged.
  5. Dose vehicle development is not included as part of this assay.
  6. The standard anticoagulant is sodium heparin. The customer can specify that another anticoagulant be used.


  1. The customer must specify:
    • any two dose routes—IV, OG, IP, or SC
    • the dose (mg/kg) of each dose route
    • the formulation instructions, if applicable
  2. The customer can request:
    • the number of replicates (typically 3)
    • different time points and an extended duration
    • that Absorption Systems determine a suitable dosing vehicle
    • that Absorption Systems prepare the dosing vehicle using the customer’s instructions
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1 thought on “Rabbit PK

  1. Hello,

    I am interested in the amount of compound typically needed to perform a Rabbit PK study (IP and OG, n=3 rabbits per each). Is there a default mg/kg dose you use for these studies?


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