Simultaneous Metabolite Profiling and CYP Induction in Plated Cryopreserved Human Hepatocytes with Omeprazole as a Model Compound

Simultaneous Metabolite Profiling and CYP Induction in Plated Cryopreserved Human Hepatocytes with Omeprazole as a Model Compound
Igor Mezine, Chris Bode, Sid Bhoopathy, Albert J. Owen, and Ismael J. Hidalgo
Absorption Systems, Exton, PA
This poster was presented at AAPS PharmSci 360, November 2018.
In vitro metabolite profiling of drug candidates, which is an integral part of the modern drug discovery and development process, is typically performed using either subcellular hepatic fractions or suspensions of cryopreserved hepatocytes. While cryopreserved hepatocytes possess a complete set of enzymatic activities, metabolism in SCH could be negatively impacted by lower activities of drug uptake transporters or, in the case of slowly metabolized drugs, be restricted due to insufficient incubation time limited by viability of SCH. Alternative approaches, based on application of plated cryopreserved hepatocytes or plated hepatic cell lines, are commercially available but more expensive. In this work, we have evaluated the applicability of CYP induction-certified PCH to study metabolism in parallel with standard CYP induction experiments, using omeprazole as a model compound.

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