Absorption Systems offers the isolated, perfused rat liver model, a powerful and highly physiological model that provides information on hepatic extraction, metabolism, and biliary efflux. Rather than setting up and running the model yourself, leave the perfusing to us!
We cannulate the bile duct, portal vein, and inferior vena cava while the anesthetized rat is still alive, prior to transferring the liver to a chamber where it is perfused with buffer and bile is collected in real time. The liver is anatomically and functionally intact, including bile production. Using specific inhibitors, we can deduce the enzyme(s) responsible for metabolism of drugs in the rat, as well as the transporter(s) involved in biliary excretion.
Use of this Model:
Rank-order compounds in terms of biliary efflux.
Assess the extent of hepatic involvement in first-pass extraction.
Evaluate drug metabolism in a physiological setting without systemic complications.
Determine the potential for transporter-based drug-drug interactions in the liver.
Identify the transporters involved in hepatic uptake and/or biliary efflux.
Investigate the cholestatic potential of test compounds