Characterization of Retinal Ganglion Cell and Optic Nerve Phenotypes Caused by Sustained Intracranial Pressure Elevation in Mice

Characterization of Retinal Ganglion Cell and Optic Nerve Phenotypes Caused by Sustained Intracranial Pressure Elevation in Mice

Guofu Shen1, Schuyler Link1, Sandeep Kumar1, Derek M. Nusbaum1,2, Dennis Y. Tse 1,3, Yingbin Fu1,2, Samuel M. Wu1,2 & Benjamin J. Frankfort1,2

“Elevated intracranial pressure (ICP) can result in multiple neurologic sequelae including vision loss.  Inducible models of ICP elevation are lacking in model organisms, which limits our understanding of the mechanism by which increased ICP impacts the visual system. We adapted a mouse model for the sustained elevation of ICP and tested the hypothesis that elevated ICP impacts the optic nerve and retinal ganglion cells (RGCs)….”

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Dr. Sandeep Kumar, one of the authors on this paper, currently works as Senior Scientist in the Ophthalmology department of Absorption System Inc, San Diego. He Serves as Principal Investigator or Study Director for preclinical ophthalmic studies and interface with different teams including in-vivo scientists, QC/QA, Account Managers/Sales. Dr. Kumar has 10 years of experience working in ocular research field. He worked at Karolinska Institute, Sweden; Moran Eye Center, University of Utah; Cullen Eye Institute at Baylor College of medicine, USA. Dr. Kumar has made significant contributions in creating animal models for eye disease research. He has transgenically generated and characterized a mouse model for Polypoidal Choroidal Vasculopathy (PCV), a variant of Age Related Macular Degeneration (AMD), which mimic human PCV condition. Dr. Kumar also developed novel methods to characterize the pathology in the choroid/retina vasculature. In a collaborative work, he answered a long debated question in the field on the existence of Visual Pigment as a monomer or dimer? and showed for the first time that “inside a living organism” rhodopsin exists as a dimer. In addition, He has shown therapeutic implications of CRISPR/dCas9 technology by delivering Lentivirus vectors containing CRISPR dCas9 & VEGF sgRNAs in laser induced CNV mouse model and showed that VEGF levels can be significantly down regulated in long term. Dr. Kumar’s scientific papers and presentations has been published in prestigious journals such as Proceedings of the National Academy of Science USA, Circulation Research, Investigative Ophthalmology Vision Science, Clinical genetics, BBA and Molecular and Cellular Biochemistry etc. His research interest are to develop animal models for ocular diseases and use them to develop new treatment strategies.

Original Source: www.nature.com/scientificreports/