Cell Based Permeability
Different cell lines are used as in vitro models for different purposes.
Caco-2 cell monolayers predict not only intestinal absorption but also drug-drug interactions involving transporters. Likewise, MDR1-MDCK cell monolayers are used to predict drug-drug interactions involving the efflux transporter P-glycoprotein as well as blood-brain barrier (BBB) penetration.
Other ExpressPlus™ assays utilizing the same test format identify substrates and inhibitors of transporters. Taken together, these studies help identify compounds with drug-like properties. The fixed protocols and rapid turnaround time make them ideal for screening large numbers of compounds early in drug discovery. At a later stage, when the same tests are run as custom assays, including test conditions optimized for a given compound, additional bioanalytical rigor, etc., you can count on getting the same results. That’s because the Absorption Systems philosophy is, wherever possible, to use the same rigorously validated test systems throughout the drug development continuum.
We perform tissue-based studies. These models can be used in early-stage preclinical studies to compare different formulations of your drug or can be used post-marketing to evaluate new formulations and determine the feasibility of various dose routes.