ExpressPlus Metabolic Stability in Liver MicrosomesThis assay is used to detect expected metabolites and determine the percent remaining of a test compound incubated with human, rat, dog, primate or mouse liver microsomes in the presence of NADPH Catalog number EA401-B Required from Customer Deliverables Substrate Assay System Assay Conditions Assay QC Notes
  1. The results from this assay are sent to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. This assay does not provide stability information of test compound in liver microsomes without NADPH.
  3. Percent remaining at each time point is calculated from the peak area response ratio of test compound (or control) divided by the peak area response ratio of the 0-minute sample.
  4. In vitro intrinsic clearance (CLint in-vitro) = k/P (mL/min·mg protein), where k is the elimination rate constant (min-1) and P is the protein concentration (mg/mL).