Induction of CYP1A2, 2B6, and 3A In Fresh Human HepatocytesThis assay is used to assess the potential of a test compound to induce CYP1A2, CYP2B6, and CYP3A in fresh human hepatocytes Catalog number EA451 Required from Customer Deliverables Substrate Assay System Assay Conditions Assay QC Notes
  1. CYP-specific inducers as positive controls
CYP Isoform Positive Control (CYP Inducer)
1A2 Omeprazole, 50 µM
2B6 Phenobarbital, 1000 µM
3A Rifampicin, 50 µM
  1. Cell viability is determined by measuring the concentration of formazan dye converted from MTS by metabolically active hepatocytes based on absorbance at 492 nm.