ExpressPlus P-gp Substrate Assessment in Caco-2 Cells (+/- Inhibitor)

This assay is used to determine the interaction of a test compound with P-gp using Caco-2 cell monolayers with and without a P-gp inhibitor

Catalog number EA253

Required from Customer

Deliverables

Substrate

Assay System

Assay Conditions

Assay QC

Notes

  1. The results from this assay are sent to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. Monolayer integrity will be verified by a pre-experimental TEER.
  3. This screening assay does not provide definitive information about the P-gp compound interactions or the apparent Km.
  4. Assay conditions with the inhibitor include:
  5. Assay conditions without the inhibitor include: