ExpressPlus OATP1B3 Inhibition Assessment Using Transfected Cells

This OATP1B3 transporter assay is used to screen for inhibition of OATP1B3 by a test compound in OATP1B3-transfected HEK293 cells at a single concentration

Catalog number EA266

Required from Customer



Assay System

Assay Conditions

Assay QC


  1. The results from this assay are provided to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. The solubility of the test compound in Hanks’ buffer containing < 0.8% DMSO must be greater than the test concentration. If the solubility of the test compound is unavailable, Absorption Systems can conduct a solubility assessment at an additional charge.
  3. Percent inhibition is calculated as 100 x [1-(IR1B3-IRVC)TC / (IR1B3 - IRVC)0], where IR1B3 is the influx rate of atorvastatin in OATP1B3-transfected cells, IRVC is the influx rate in vector control cells, “TC”  means in the presence of test compound, and “0” means in the absence of test compound.


  1. The customer can request: