ExpressPlus OATP1B1 Inhibition Assessment Using Transfected Cells

This assay is used to screen for inhibition of OATP1B1 by a test compound in OATP1B1-transfected HEK293 cells at a single concentration

Catalog number EA265

Required from Customer

Deliverables

Substrate

Assay System

Assay Conditions

Assay QC

Notes

  1. The results from this assay are provided to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. The solubility of the test compound in Hanks’ buffer containing < 0.8% DMSO must be greater than the test concentration. If the solubility of the test compound is unavailable, Absorption Systems can conduct a solubility assessment at an additional charge.
  3. Percent inhibition is calculated as 100 x [1-(IR1B1-IRVC)TC / (IR1B1 - IRVC)0], where IR1B1 is the influx rate of atorvastatin in OATP1B1-transfected cells, IRVC is the influx rate in vector control cells, “TC”  means in the presence of test compound, and “0” means in the absence of test compound.

Options

  1. The customer can request: