ExpressPlus Metabolic Stability in Liver S9 Fraction

This assay is used to determine the percent remaining of a test compound incubated with liver S9 fraction (mouse, rat, dog, monkey, or human) in the presence of cofactors

Catalog number EA424-A

Required from Customer

Deliverables

Substrate

Assay System

Assay Conditions

Assay QC

Notes

  1. The results from this assay are sent to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. This assay does not provide stability information of test compound in liver S9 incubations without cofactors.
  3. Percent remaining at each time point is calculated from the peak area response ratio of test compound (or control) divided by the peak area response ratio of the 0-minute sample.
  4. In vitro intrinsic clearance (CLint in-vitro) = k/P (mL/min·mg protein), where k is the elimination rate constant (min-1) and P is the protein concentration (mg/mL).