ExpressPlus BSEP Inhibition Assessment Using Vesicles

This BSEP transporter assay is used to screen for inhibition of BSEP by a test compound in hBSEP-expressing vesicles at a single concentration

Catalog number EA277

Required from Customer

Deliverables

Substrate

Assay System

Assay Conditions

Assay QC

Notes

  1. The results from this assay are provided to the customer in the ExpressPlus report format, which may include graphical representations of data and comparison with historical data for reference compounds.
  2. The solubility of the test compound in Hepes Influx Buffer must be greater than the test concentration. If the solubility of the test compound is unavailable, Absorption Systems can conduct a solubility assessment at an additional charge.
  3. Percent inhibition is calculated as 100 x [1-(IRATP-IRAMP)TC / (IRATP – IRAMP)0], where IRATP is the influx rate of 3H-taurocholic acid in hBSEP-expressing vesicles in the presence of ATP, IRAMP is the influx rate of 3H-taurocholic acid in hBSEP-expressing vesicles in the presence of AMP, “TC”  means in the presence of test compound, and “0” means in the absence of test compound.

Options

  1. The customer can request: