Binding and Distribution

Essential In Vitro Assays for Prediction of In Vivo Drug Distribution

Knowledge of the basic binding and distribution properties of a test compound is critical in choosing the right tools and experimental conditions to further evaluate, and understand, a given compound’s ADMET characteristics. For example, evaluation of a compound’s compartmental distribution in blood (e.g., plasma protein binding, blood-to-plasma partitioning, etc.) at an early stage of development helps the sponsor determine the appropriate sample matrix (whole blood vs. plasma, for instance) to evaluate pharmacokinetics of the compound in animal models. Typical approaches for these tests are inexpensive and do not require complicated test systems.

Absorption Systems implements industry-standard practices with customizable assay conditions in evaluating a compound’s binding and distribution properties. A wide range of assay variables such as: tissue (brain, blood, etc.), protein (e.g. total plasma proteins vs. albumin), species, anticoagulant, incubation time, and others can be customized to determine the compound’s properties.

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Binding and Distribution Assays
Plasma Protein Binding (Equilibrium Dialysis or Ultrafiltration)
Fraction Unbound in Brain
Blood-to-Plasma Partitioning

Species: Mouse, Rat, Dog, Monkey, Human, others available upon request.